Lab4 and Cholesterol Study

The anti-cholesterolaemic effect of a consortium of probiotics: An acute study in C57BL/6J mice (Part 1)

SUMMARY

In the present study, assessment of the cholesterol lowering capabilities of Lab4 was made in vitro prior to its inclusion, in combination with Lactobacillus plantarum CUL66, in a short-term feeding study with C57BL/6J mice on a high fat diet.
This in vitro study demonstrated the cholesterol lowering potential of Lab4 probiotics by their ability to inhibit the process of cholesterol uptake in the gut intestine.

Aim

To examine the cholesterol lowering abilities of Lab4 probiotics in an in vitro model of the intestinal epithelium.

Method

• Cholesterol lowering ability and bile salts hydrolase (BSH) activity in Lab4 probiotics was assessed using in vitro models
• The effect of Lab4 probiotics on cholesterol transport was assessed in an in vitro Caco-2 cells intestinal model

Results

Cholesterol removal

• Cholesterol levels in bacterial culture media were lowered in the presence of Lab4 probiotic mixture (#P=0.076)

Bile salt hydrolase activity

• A white precipitate was observed when Lab4 probiotic mixture was grown in the presence of bile salts, which is indicative of BSH activity.

Cholesterol transport

• Lab4 probiotic mixture significantly reduced the expression of cholesterol transporter NPC1L1, which is critical for the uptake of cholesterol (**P<0.001)

• Lab4 probiotic mixture significantly reduced the efflux of intracellular cholesterol from intestinal epithelial cells into the basolateral (tissue) compartment (*P=0.004)

• Lab4 probiotic mixture significantly reduced the expression of cholesterol transporter ABCA-1, which is involved in the efflux of intracellular cholesterol to the basolateral (tissue) compartment (*P<0.01)

Summary Proposed Mechanism of Action

1. CHOLESTEROL REMOVAL [cholesterol is bound or metabolised by Lab4 and subsequently excreted in faeces]
2. BILE SALT HYDROLASE ACTIVITY [deconjugation (modifying) of bile acids leads to their reduced re-absorption and places an increased demand on the liver to synthesize more bile acids from circulating cholesterol in blood to replenish intestinal bile acids lost in faeces]
3. REDUCTION OF CHOLESTEROL TRANSPORT ACROSS THE INTESTINAL EPITHELIUM [3a – reduced expression of the NCP1L1 cholesterol transporter; 3b- reduction in the efflux of intracellular cholesterol to the basolateral compartment (tissue) and reduced expression of the ABCA-1 cholesterol transporter; 3c – no change on the intracellular cholesterol efflux back into the apical (intestinal lumen) compartment]

Conclusion

Lab4 probiotics showed promising cholesterol lowering potential.

Reference

Michael DR et al. 2017 The anti-cholesterolaemic effect of a consortium of probiotics: An acute study in C57BL/6J mice. Scientific Reports 7:2883
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